Electrophysiological and behavioral responses of Myzus persicae to alarm pheromone and tobacco plant volatiles

doi:10.7679/j.issn.2095-1353.2020.01.019

Abstract

Abstract:

[Objectives] To provide information to inform the choice of pheromones and volatile compounds for the environmentally-friendly control of crop damage by this species, the effects of tobacco volatiles and an alarm pheromone on the behavior of Myzus persicae were measured. [Methods] Electrophysiological responses of winged and wingless M. persicae to 1, 10 and 100 ng concentrations of the aphid alarm pheromone ((E)-β-farnesene) and four volatile tobacco plant compounds (6-methyl-5-hepten-2-one, butyl acetate, (E)-β-ocimene and nonanal) were recorded by EAG and the behavioral responses of individual aphids to these compounds were recorded in a Y-tube olfactometer. [Results] Aphid alarm pheromone and the four tobacco volatile compounds tested all elicited EAG responses in both winged and wingless aphids. Winged aphids were more sensitive to the alarm pheromone than wingless ones, whereas wingless aphids were more sensitive to butyl acetate and (E)-β-ocimene. Both 10 ng and 100 ng concentrations of the alarm pheromone repelled both winged and wingless aphids, but the 1ng concentration only repelled wingless aphids. One hundred nanogram concentrations of 6-methyl-5-hepten-2-one, butyl acetate and (E)-β-ocimene were significantly attractive to both winged and wingless aphids. A concentration of 10 ng butyl acetate attracted both winged and wingless aphids, but the same concentration of 6-methyl-5-hepten-2-one only attracted wingless ones. [Conclusion] The alarm pheromone has a stronger repellent effect on wingless aphids than winged aphids, and 6-methyl-5-hepten-2-one, butyl acetate and (E)-β-ocimene are attractive to both winged and wingless aphids.

Key words: Myzus persicae, tobacco plant volatiles, alarm pheromones, EAG, behavioral response

Bo WEI, Bao-Yuan CHEN, Xiao-Jiao ZHANG, Cheng-Mei WEI, Ying JING, Cheng-Yun LI, Wen-Xia DONG. Electrophysiological and behavioral responses of Myzus persicae to alarm pheromone and tobacco plant volatiles[J]. Chinese Journal of Applied Entomology, 2020, 57(1): 173-180.

Figures/Tables 9

Table 1 EAG response of winged and wingless Myzus persicae to aphid alarm pheromone and tobacco volatile compounds

化合物 Compounds	处理/对照 Treatment/Control	EAG反应相对值 The EAG relative potential
化合物 Compounds	T/CK	有翅蚜 Winged M. persicae	无翅蚜 Wingless M. persicae
反-β-法尼烯 (E)-β-Farnesene	T	1.047±0.094^**	0.511±0.016^**
反-β-法尼烯 (E)-β-Farnesene	CK	0.029±0.009	0.030±0.004
6-甲基-5-庚烯-2-酮 6-Methyl-5-hepten-2-one	T	1.334±0.042^*	1.451±0.048^*
6-甲基-5-庚烯-2-酮 6-Methyl-5-hepten-2-one	CK	0.013±0.002	0.068±0.009
壬醛 Nonanal	T	0.727±0.012^*	0.864±0.060^**
壬醛 Nonanal	CK	0.028±0.004	0.034±0.006
乙酸正丁酯 Butyl acetate	T	0.971±0.034^*	1.363±0.062^*
乙酸正丁酯 Butyl acetate	CK	0.017±0.002	0.031±0.002
反-β-罗勒烯 (E)-β-Ocimene	T	0.484±0.094^*	0.873±0.029^*
反-β-罗勒烯 (E)-β-Ocimene	CK	0.055±0.001	0.019±0.006

Fig. 1 The comparison of EAG relative responses of wingless and winged Myzus persicae to tobacco volatiles and aphid alarm pheromone Data are mean± SE. Histograms with * indicate significant difference among the EAG relative potentials of winged and wingless M. persicae to the same compound by t-test (P<0.05).

Fig. 2 The comparison of EAG relative responses of wingless Myzus persicae to tobacco volatiles and aphid alarm pheromone Data are mean± SE. Histograms with the differerent lowercase letters indicate significant difference among the EAG relative potentials of winged and wingless M. persicae to different compounds by Tukey’s test (P<0.05). The same as Fig.3.

Fig. 3 The comparison of EAG relative responses of winged Myzus persicae to tobacco volatiles and aphid alarm pheromone

Fig. 4 Olfactory responses of Myzus persicae to (E)-β-Farnesene in a Y-tube olfactometer * indicates significant difference at P<0.05, ** indicates extremely significant difference at P<0.01, ns indicates no significant difference by c2-test. The same below.

Fig. 5 Olfactory responses of Myzus persicae to 6-Methyl-5-hepten-2-one in a Y-tube olfactometer

Fig. 6 Olfactory responses of Myzus persicae to butyl acetate in a Y-tube olfactometer

Fig. 7 Olfactory responses of Myzus persicae to (E)-β-Ocimene in a Y-tube olfactometer

Fig. 8 Olfactory responses of Myzus persicae to nonanal in a Y-tube olfactometer

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