Effects of trehalose on the physiological and biochemical characteristics of rice, including resistance to the brown planthopper (BPH), Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)

doi:10.7679/j.issn.2095-1353.2020.083

Abstract

Abstract:

[Objectives] Trehalose is involved in the response and adaptation of plants to stress. This article aims to clarify the effect of trehalose on the physiological and biochemical characteristics of rice and resistance to the brown planthopper (BPH), Nilaparvata lugens (St?l) (Hemiptera: Delphacidae), which will help to fully explore the potential role of trehalose on rice and provide reference and basis for subsequent research. [Methods] The effects of trehalose on the physiological and biochemical characteristics of rice, including its resistance to the brown planthopper were investigated after treatment with 10 mmol?L ^–1 and 50 mmol?L ^–1 trehalose; specifically, the activity of the enzymes superoxide dismutase (SOD), peroxidase (POD) and malondialdehyde (MDA), and soluble sugar content. In addition, the functional plant loss index (FPLI) after BPH had fed on rice plants and the effects of trehalose treatment on BPH feeding behavior were investigated with an electrical penetration graph (EPG). [Results] The results showed that after applying trehalose at a concentration of 10 mmol·L ^–1 and 50 mmol·L ^–1, the POD activity and soluble sugar content increased significantly, the MDA content decreased significantly, and the function loss index of rice plants was significantly improved. EPG results found that trehalose treatment significantly increased the duration of the N4 wave. [Conclusion] Although appropriate levels of trehalose can improve the resistance of rice to abiotic stress, it does not improve the resistance of rice to BPH.

Key words: trehalose, rice, physiology and biochemistry, brown planthopper, rice resistance to pest

Li-Tong SUN, Ling FENG, Zi-Rui LIU, Xiao-Wei XU, Jing-Lan LIU. Effects of trehalose on the physiological and biochemical characteristics of rice, including resistance to the brown planthopper (BPH), Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)[J]. Chinese Journal of Applied Entomology, 2020, 57(4): 814-822.

Figures/Tables 4

Table 1 Identification criteria of rice resistance to brown planthopper at seedling stage (Wu et al., 1986)

苗期受害等级（级） Injury grade at seedling stage (level)	国际水稻研究所标准 The standard of International Rice Research Institute
0	未受害 Unharmed
1	受害很轻 Victimization is very light
3	多数植株第1、第2叶变黄 The first and second leaves of most plants turn yellow
5	植株明显变黄和矮化,或有半数以上植株枯萎、死亡 Plants become noticeably yellow and dwarfed, or more than half of the plants wither and die
7	半数以上植株枯萎死亡,其余植株严重矮化,濒于死亡 More than half of the plants withered and died, and the remaining plants were severely dwarfed and were near death
9	所有植株死亡 All plants die

Fig. 1 Effects of exogenous trehalose on physiological and biochemical characteristics of rice A. Malondialdehyde (MDA); B.Superoxide dismutase (SOD); C. Peroxidase (POD); D. Soluble sugar. Data are means±SE. Histograms with the different letters indicate significant difference at the 0.05 level by LSD test. The same below.

Fig. 2 Average injury scale and plant function loss index of rice after fed by BPH A. Average injury scale; B. Functional plant loss index.

Fig. 3 EPG analysis of BPH feeding behavior in rice treated with different concentrations of trehalose NP: The duration of non probing stage; N1: The number of probing; N2: The duration of trocar movement in vascular bundle; N3: The duration of phloem extracellular movement; N4: The duration of sucking sap in phloem; N5: The duration of sucking xylem sap.

References 40

[1]	Abebe T, Guenzi AC, Martin B, Cushman JC , 2003. Tolerance of mannitol-accumulating transgenic wheat to water stress and salinity. Plant Physiology, 131(4):1748-1755. doi: 10.1104/pp.102.003616 URL pmid: 12692333
[2]	Award M , 1990. Prospect of microencapusating red phosphorus. Chemistry Industry, ( 8):19-21.
[3]	Beauchamp C, Fridovich I , 1971. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry, 44(1):276-287. doi: 10.1016/0003-2697(71)90370-8 URL pmid: 4943714
[4]	Chen JM , 2004. Tolerance of rice varieties to brown planthopper damage and its physiological mechanism. Doctoral dissertation. Zhejiang: Zhejiang University.
	[ 陈建明 , 2004. 水稻品种对褐飞虱为害的耐性及其生理机制研究. 博士学位论文. 浙江: 浙江大学.]
[5]	Chen QY , 2017. Study on enhancement of tobacco resistance to common mosaic disease by exogenous trehalose. Master dissertation. Henan: Henan Agricultural University.
	[ 陈芊伊 , 2017. 外源海藻糖增强烟草对普通花叶病抗性的研究. 硕士学位论文. 河南: 河南农业大学.]
[6]	Cheng JA, Zhu ZR , 2006. Analysis on the key factors causing the outbreak of brown planthopper in Yangtze Area, China in 2005. Plant Protection, 32(4):1-4.
[7]	Crowe JH, Carpenter JF, Crowe LM , 1998. The role of vitrification in anhydrobiosis. Annual Review of Physiology, 60(1):73-103. doi: 10.1146/annurev.physiol.60.1.73 URL
[8]	Elbein AD , 1974. The metabolism of α, α-trehalose. Advances in Carbohydrate Chemistry and Biochemistry, ( 30):227-256.
[9]	Gill SS, Tuteja N , 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 48(12):909-930. doi: 10.1016/j.plaphy.2010.08.016 URL
[10]	Goddijn O, Dun K , 1999. Trehalose metabolism in plants. Trends in Plant Science, 4(8):315-319. doi: 10.1016/s1360-1385(99)01446-6 URL pmid: 10431221
[11]	Goddijn O, Smeekens S , 1998. Sensing trehalose biosynthesis in plants. Plant Journal, 14(2):143-146. doi: 10.1046/j.1365-313x.1998.00140.x URL pmid: 9628011
[12]	Govind SR, Sudisha J, Mostafa A, Shetty HS, Tran LP , 2016. Exogenous trehalose treatment enhances the activities of defense-related enzymes and triggers resistance against downy mildew disease of pearl millet. Frontiers in Plant Science, ( 7):1593.
[13]	Hibino H , 1996. Biology and epidemiology of rice viruses. Annual Review of Phytopathology, 34(1):249-274. doi: 10.1146/annurev.phyto.34.1.249 URL
[14]	Jing P, Bai SF, Liu F , 2013. Preliminary study on spike potential waveform of feeding behavior of Laodelphax striatellus. Chinese Journal of Applied Entomology, 50(3):758-763.
	[ 荆裴, 白素芬, 刘芳 , 2013. 灰飞虱取食行为刺吸电位波形的初步研究. 应用昆虫学报, 50(3):758-763.]
[15]	Lei H, Xu RM , 1998. EPG study on feeding behavior of whitefly in greenhouse. Acta Entomologica Sinica, 41(2):113-123.
	[ 雷宏, 徐汝梅 , 1998. 温室白粉虱取食行为的刺探电位(EPG) 研究. 昆虫学报, 41(2):113-123.]
[16]	Li L, Huang QC, Qin GY , 2003. Research progress of trehalose in improving plant stress resistance. Bulletin of Biology, 38(6):6-7.
	[ 李莉, 黄群策, 秦广雍 , 2003. 海藻糖在提高植物抗逆性方面的研究进展. 生物学通报, 38(6):6-7.]
[17]	Li MY, Cao ZX, Yu XY , 2006. Effects of low temperature exercise on protective enzymes of cucumber seedlings under cold stress. Acta Agriculturae Boreali-occidentalis Sinica, 15(1):160-164.
	[ 李明玉, 曹辰兴, 于喜艳 , 2006. 低温锻炼对冷胁迫下黄瓜幼苗保护性酶的影响. 西北农业学报, 15(1):160-164.]
[18]	Li YY, Liang GJ, Li YH, Ye QS , 2004. Effect of exogenous betaine on cold resistance of cucumber seedlings. Plant Physiology Communications, 40(6):673-676.
	[ 李芸瑛, 梁广坚, 李永华, 叶庆生 , 2004. 外源甜菜碱对黄瓜幼苗抗冷性的影响. 植物生理学通讯, 40(6):673-676.]
[19]	Liu JX, Wang X, Li BP , 2010. Effects of exogenous nitric oxide donor SNP on ascorbic acid-glutathione cycle in ryegrass seedlings leaves under NaCl stress. Acta Prataculturae Sinica, 19(2):82-88. doi: 10.11686/cyxb20100212 URL
	[ 刘建新, 王鑫, 李博萍 , 2010. 外源一氧化氮供体SNP对NaCl胁迫下黑麦草幼苗叶片抗坏血酸-谷胱甘肽循环的影响. 草业学报, 19(2):82-88.]
[20]	Long ZL , 2014. The role of trehalose in plant response and adaptation to stress. Journal of Anhui Agricultural Sciences, 449(16):4956-4959.
	[ 龙正龄 , 2014. 海藻糖在植物对逆境胁迫响应与适应中的作用. 安徽农业科学, 449(16):4956-4959.]
[21]	Luo C, Yue M, Xu HF, Zhang ZL , 2005. Application and progress of EPG technology in entomology research. Acta Entomologica Sinica, 48(3):437-443.
	[ 罗晨, 岳梅, 徐洪富, 张芝利 , 2005. EPG技术在昆虫学研究中的应用及进展. 昆虫学报, 48(3):437-443.]
[22]	Ma C, Wang ZQ, Kong BB, Lin TB , 2013. Exogenous trehalose differentially modulate antioxidant defense system in wheat callus during water deficit and subsequent recovery. Plant Growth Regulation, 70(3):275-285. doi: 10.1007/s10725-013-9799-2 URL
[23]	Ma GS, Yang J, Lian H, Wang RH, Wu X , 2010. Effects of trehalose on osmotic regulators and enzyme activities of tomato under salt stress. Northern Horticulture, ( 6):59-61.
	[ 马光恕, 杨瑾, 廉华, 王茹华, 吴瑕 , 2010. 盐胁迫下海藻糖对番茄渗透调节物及酶活性的影响. 北方园艺, ( 6):59-61.]
[24]	Madan S , 2015. Trehalose mitigates heat stress-induced damages in wheat seedlings. Journal of Wheat Research, 7(1):74-78.
[25]	Miao J, Han BY , 2008. DC-EPG analysis on effect of tea plant induced by methyl salicylate against feeding of tea green leafhopper. Acta Phytophylacica Sinica, 35(2):143-147.
[26]	Olivier F, Linda B, Anthony Q, Rajbir SS, Christophe C , 2010. Trehalose and plant stress responses: Friend or foe? Trends in Plant Science, 15(7):409-417. doi: 10.1016/j.tplants.2010.04.004 URL
[27]	Pang CP, Ye L, Ma J, Lu T, Yang ZY, Qi MF , 2017. Regulation of trehalose on photosynthesis of tomato seedling leaves under high temperature. Jiangsu Agricultural Sciences, 45(21):151-154.
	[ 庞椿朋, 叶亮, 马健, 路涛, 杨宗艺, 齐明芳 , 2017. 海藻糖对高温下番茄幼苗叶片光合作用的调控作用. 江苏农业科学, 45(21):143-146.]
[28]	Peng GS , 2011. Preliminary study on resistance mechanism of different rice varieties to laodelphax striatellus. Master thesis. Henan: Henan Normal University.
	[ 彭高松 , 2011. 不同水稻品种对灰飞虱抗性机理的初步研究. 硕士学位论文. 河南:河南师范大学.]
[29]	Quan RL, Wang QL, Ma HY, Fu D, Huo EW, Shen GH, Guo GY , 2015. Effects of drought on rice growth and development and research progress on drought resistance. China Seed Industry, ( 9):12-14.
	[ 全瑞兰, 王青林, 马汉云, 扶定, 霍二伟, 沈光辉, 郭桂英 , 2015. 干旱对水稻生长发育的影响及其抗旱研究进展. 中国种业, ( 9):12-14.]
[30]	Simms EL , 2000. Defining tolerance as a norm of reaction. Evolutionary Ecology, 14(4):563-570. doi: 10.1023/A:1010956716539 URL
[31]	Strauss SY, Agrawal AA , 1999. The ecology and evolution of plant tolerance to herbivory. Trends in Ecology & Evolution, 14(5):179-185. doi: 10.1016/s0169-5347(98)01576-6 URL pmid: 10322530
[32]	Velusamy R, Heinrichs EA , 1986. Electronic monitoring of feeding behavior of Nilaparvata lugens(Homoptera: Delphacidae) on resistant and susceptible rice cultivars. Environmental Entomology, 15(3):678-682. doi: 10.1093/ee/15.3.678 URL
[33]	Wiemken A , 1990. Trehalose in yeast, stress protectant rather than reserve carbohydrate. Antonie van Leeuwenhoek, 58(3):209-217. doi: 10.1007/BF00548935 URL pmid: 2256682
[34]	Wu GR, Tao LY, Chen FY, Hu GW , 1986. Discussion on screening methods of rice resistance to white-backed planthopper. Acta Entomologica Sinica, 29(4):453-455.
	[ 巫国瑞, 陶林勇, 陈福云, 胡国文 , 1986. 水稻对白背飞虱抗性筛选方法的探讨. 昆虫学报, 29(4):453-455.]
[35]	Xiao YF, Zhang CZ, Gu ZY , 2001. Resistance mechanism of rice varieties to white-backed planthopper. Acta Phytophylacica Sinica, 28(3):198-202.
	[ 肖英方, 张存政, 顾正远 , 2001. 水稻品种对白背飞虱的抗性机理. 植物保护学报, 28(3):198-202.]
[36]	Xu T, Zhou CY, Zhou C, Zhao S, Wu LL, Tan KF , 2014. Effects of trehalose on antioxidant system of muskmelon seedlings under salt stress. Northern Horticulture, ( 19):28-30.
	[ 徐婷, 周传余, 周超, 赵索, 武琳琳, 谭可菲 , 2014. 海藻糖对盐胁迫下薄皮甜瓜幼苗抗氧化系统的影响. 北方园艺, ( 19):28-30.]
[37]	Xue J, Bao YY, Li BL, Cheng YB, Peng ZY, Liu H, Xu HJ, Zhu ZR, Lou YG, Cheng JA, Zhang CX , 2010. Transcriptome analysis of the brown planthopper Nilaparvata lugens. PLoS ONE, 5(12):e14233. doi: 10.1371/journal.pone.0014233 URL pmid: 21151909
[38]	Yang SJ , 2005. Resistance of hybrid offspring of medicinal wild rice to Cnaphalocrocis medinalis. Journal of Anhui Agricultural Sciences, 33(4):570.
	[ 杨士杰 , 2005. 药用野生稻杂交后代对稻纵卷叶螟的抗性. 安徽农业科学, 33(4):570.]
[39]	Yang SH , 2006. Effects of salt stress on plants and the mechanism of salt tolerance. World Sci-tech R＆D, 28(4):70-76.
[40]	Zhao KF , 1993. Salt Tolerance Physiology of Plants. Beijing: China Science and Technology Press. 25-30.
	[ 赵可夫 , 1993. 植物抗盐生理. 北京: 中国科技出版社. 25-30.]