Chinese Journal of Applied Entomology ›› 2020, Vol. 57 ›› Issue (4): 833-840.doi: 10.7679/j.issn.2095-1353.2020.085
• Special Column • Previous Articles
Teng-Fei SHI(), An-Ran WANG(), Yu-Jie ZHU, Lin-Sheng YU***()
Received:
2019-12-27
Accepted:
2020-05-11
Online:
2020-07-27
Published:
2020-09-02
Contact:
Lin-Sheng YU
E-mail:stf0623@163.com;aimyon01@163.com;yulinshengahau@163.com
Teng-Fei SHI, An-Ran WANG, Yu-Jie ZHU, Lin-Sheng YU. Sublethal effects of sulfoxaflor on the expression of detoxification, immune and memory related, genes in the honeybee (Apis mellifera ligustica)[J]. Chinese Journal of Applied Entomology, 2020, 57(4): 833-840.
基因 Genes | 引物序列(5¢-3¢) Primer sequences (5¢-3¢) | 基因 Genes | 引物序列(5¢-3¢) Primer sequences (5¢-3¢) |
---|---|---|---|
CYP9Q1 | F: TCGAGAAGTTTTTCCACCG | Apidaecin | F: TTTTGCCTTAGCAATTCTTGTTG |
R: CTCTTTCCTCCTCGATTG | R: GAAGGTCGAGTAGGCGGATCT | ||
CYP9Q2 | F: GATTATCGCCTATTATTACTG | CYP9S1 | F: CTAATTTTCGCGTTCCCAAA |
R: GTTCTCCTTCCCTCTGAT | R: CTCCCGTTACGTTTGTCGAT | ||
CYP9Q3 | F: GTTCCGGGAAAATGACTAC | CYP4G11 | F: CAAAATGGTGTTCTCCTTACCG |
R: GGTCAAAATGGTGGTGAC | R: ATGGCAACCCATCACTGC | ||
CYP6AS5 | F: CAGGCTCTCCCCAATATTCA | PKA | F: AAGACTATTGAAGTCGGTGACA |
R: TCGATGGGCTCATTTTTCTC | R: CCTATCAAGGCCCCACCAAA | ||
Abaecin | F: TGTCGGCCTTCTCTTCATGG | NMDAR1 | F: GTATTTCCGTCGCCAAGTC |
R: TGACCTCCAGCTTTACCCAAA | R: TGTAAACCAATCCCATAGCCA | ||
Hymenoptaecin | F: ATATCCCGACTCGTTTCCGA | GluRA | F: ACTCTGTTCGTCTGTGGGGTC |
R: TCCCAAACTCGAATCCTGCA | R: TTCGTTAGAAGGGCAGCGTA | ||
Defensin | F: TGCGCTGCTAACTGTCTCAG | RpS5 | F: AATTATTTGGTCGCTGGAATTG |
R: AATGGCACTTAACCGAAACG | R: TAACGTCCAGCAGAATGTGGTA |
Table 1 The sequences of forward and reverse primers of genes used in the present study
基因 Genes | 引物序列(5¢-3¢) Primer sequences (5¢-3¢) | 基因 Genes | 引物序列(5¢-3¢) Primer sequences (5¢-3¢) |
---|---|---|---|
CYP9Q1 | F: TCGAGAAGTTTTTCCACCG | Apidaecin | F: TTTTGCCTTAGCAATTCTTGTTG |
R: CTCTTTCCTCCTCGATTG | R: GAAGGTCGAGTAGGCGGATCT | ||
CYP9Q2 | F: GATTATCGCCTATTATTACTG | CYP9S1 | F: CTAATTTTCGCGTTCCCAAA |
R: GTTCTCCTTCCCTCTGAT | R: CTCCCGTTACGTTTGTCGAT | ||
CYP9Q3 | F: GTTCCGGGAAAATGACTAC | CYP4G11 | F: CAAAATGGTGTTCTCCTTACCG |
R: GGTCAAAATGGTGGTGAC | R: ATGGCAACCCATCACTGC | ||
CYP6AS5 | F: CAGGCTCTCCCCAATATTCA | PKA | F: AAGACTATTGAAGTCGGTGACA |
R: TCGATGGGCTCATTTTTCTC | R: CCTATCAAGGCCCCACCAAA | ||
Abaecin | F: TGTCGGCCTTCTCTTCATGG | NMDAR1 | F: GTATTTCCGTCGCCAAGTC |
R: TGACCTCCAGCTTTACCCAAA | R: TGTAAACCAATCCCATAGCCA | ||
Hymenoptaecin | F: ATATCCCGACTCGTTTCCGA | GluRA | F: ACTCTGTTCGTCTGTGGGGTC |
R: TCCCAAACTCGAATCCTGCA | R: TTCGTTAGAAGGGCAGCGTA | ||
Defensin | F: TGCGCTGCTAACTGTCTCAG | RpS5 | F: AATTATTTGGTCGCTGGAATTG |
R: AATGGCACTTAACCGAAACG | R: TAACGTCCAGCAGAATGTGGTA |
农药 Pesticide | 时间 Time | 毒力回归方程 Regression equation | LD50(95%置信区间,95% CL)(μg/蜂) |
---|---|---|---|
氟啶虫胺腈Sulfoxaflor | 48 h | y = 4.694 + 4.67x | 0.099(0.087-0.111) |
Table 2 LD50 value of sulfoxaflor to honeybee (Apis mellifera ligustica) by oral-feeding
农药 Pesticide | 时间 Time | 毒力回归方程 Regression equation | LD50(95%置信区间,95% CL)(μg/蜂) |
---|---|---|---|
氟啶虫胺腈Sulfoxaflor | 48 h | y = 4.694 + 4.67x | 0.099(0.087-0.111) |
Fig. 1 Probability of survival of honeybees (Apis mellifera ligustica) in different groups Data are mean±SE. Histograms with the same letters indicate no significant difference (P > 0.05), while with different letters indicate significant difference (P < 0.05). The same below.
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