Identification and expression characterization of UDP-glucosyltransferase genes in Rhaphuma horsfieldi

doi:10.7679/j.issn.2095-1353.2020.092

Abstract

Abstract:

[Objectives] UDP-glucosyltransferase (UGT) in insects is of particular importance for the detoxification of endogenous and exogenous toxic compounds. However, no information about RhorUGT genes of Rhaphuma horsfieldi is available. [Methods] In this study, we characterized the UGT gene family from R. horsfieldi using transcriptomics, bioinformatics, phylogenic and expression profiling analyses, and homology modeling approaches. [Results] Based on the transcriptomic data, totally 36 RhorUGT genes were identified from this beetle, 17 of which were full-length sequences. A comparative study on UGT gene numbers among different coleopteran species revealed that R. horsfieldi harbored a medium UGT number. Phylogenetic analysis indicated that RhorUGTs were clustered into ten sub-families, with a cerambycid-specific one of UGT352. Notably, these members formed by RhorUGT2/7/10/16/18/27 from UGT352 were possibly derived from gene duplications. Expression profiles showed that most of RhorUGTs were detected in tested tissues and some were specifically or highly expressed in antennae or tarsi, suggesting their diverse functions including olfaction and touch. Results by three-dimensional structures showed that α3, α4, β4 and β5 of RhorUGT17 were mainly involved in the binding of UDP-glucose. [Conclusion] This study has identified the numbers, sequence characteristics, phylogenic relationships and tissue expression characteristics of R. horsfieldi UGTs, and thus provides reference data for addressing the detoxification mechanisms of this beetle.

Key words: Rhaphuma horsfieldi, UDP-glycosyltransferase, phylogenetic analysis, expression profile, homology modeling

Zheng-Quan WANG, Ning-Na YIN, Ning ZHAO, Nai-Yong LIU. Identification and expression characterization of UDP-glucosyltransferase genes in Rhaphuma horsfieldi[J]. Chinese Journal of Applied Entomology, 2020, 57(4): 898-910.

Figures/Tables 6

Information for UGT genes in Rhaphuma horsfieldi

Comparison of UGT gene numbers of different sub-families in Coleoptera

Fig. 1 Multiple alignment of UGT amino acid sequences in Rhaphuma horsfieldi Triangle represents key catalytic residues (H, histidine and D, aspartic acid). 1, 2 and 3 represent nucleotide, phosphate and glucoside interacting residues, respectively.

Fig. 2 Phylogenetic tree of UGTs in Coleoptera

Fig. 3 Expression pattern of RhorUGT genes in different tissues of Rhaphuma horsfieldi adults FA: Female antennae; FTa: Female tarsi; FB: Female bodies without antennae and tarsi; MA: Male antennae; MTa: Male tarsi; MB: Male bodies without antennae and tarsi.

Fig. 4 Three-dimensional (3D) structure of Rhaphuma horsfieldi UGT17 A. Alignment of RhorUGT17 and HsapUGT2B7 amino acid sequences; B. 3D model of RhorUGT17 (left) and superimposition of RhorUGT17 and HsapUGT2B7 structures (right). Key residues (S, W, Q, T, H, E, D and H/Q) and UDP-glucose binding cavities of RhorUGT17 and HsapUGT2B7 are labeled on the structures, respectively. Donor binding domains (DBR1 and DBR2) are highlighted in yellow. Nt, Ct, α1-7 and β1-6 represent N-terminus, C-terminus, seven α-helixes and six β-sheets, respectively.

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