致死中浓度溴氰菊酯对茄无网蚜体内能源物质的影响 *

doi:10.7679/j.issn.2095-1353.2020.088

摘要/Abstract

摘要：

【目的】 通过LC₅₀溴氰菊酯胁迫1龄茄无网蚜Acyrthosiphon solani测定对其生长发育及能源物质的影响,为研究茄无网蚜抗药性产生提供理论基础。【方法】 通过浸渍法用LC₅₀溴氰菊酯处理1龄茄无网蚜,间隔12 h观测药剂胁迫后试虫的生长历期,分别用蒽酮法、考马斯亮蓝G-250法,香草醛试剂测定各虫龄试虫蛋白、糖类和总脂质含量并进行数据分析。【结果】 药剂胁迫下显著延长了1龄（1.44倍）、2龄（1.26倍）的发育历期（P < 0.05）;蛋白质比重在2龄（0.75倍）、3龄（0.69倍）、成蚜（1.17倍）有显著差异（P < 0.05）,其他虫龄有差异但不显著;可溶性糖比重在2龄（1.39倍）差异显著（P < 0.05）;糖原比重在1龄（3.81倍）、2龄（3.82倍）差异显著;总脂质在1-4龄均有显著差异（P < 0.05）,分别是对照组的2.04、1.34、1.25、1.30倍。【结论】 药剂胁迫对茄无网蚜的生长发育及各虫龄能源物质比重均有不同程度的影响。

关键词: 茄无网蚜, 致死中浓度, 溴氰菊酯, 生长历期, 能源物质

Abstract:

[Objectives] To investigate the effect of deltamethrin on the growth, development and energy storage of 1st instar nymphs of Acyrthosiphon solani Kaltenbach and thereby provide a theoretical basis for research pesticide resistance mechanisms in this pest. [Methods] First instar nymphs of A. solani were treated with an LC₅₀ dose of deltamethrin via the impregnation method after which the growth of successive instars was monitored every 12 h. In addition, the protein, carbohydrate and total lipid of each nymph was measured by staining these with anthrone, coomassie brilliant blue G-250 and vanillin, respectively. [Results] We found that deltamethrin significantly extended the growth period of 1st and 2nd instar nymphs by 1.44 times and 1.26 times, respectively (P < 0.05). There was also a significant difference in the specific gravity of protein between treatment and control groups in 2nd (0.74 times), 3nd (0.69 times) and 5th instar (1.17 times) nymphs. The protein content of other treated instars also differed from their respective control group, but not significantly. There was also a significant difference in the specific gravity of soluble carbohydrate between 2nd instar treatment and control groups (1.39 times). Glycogen differed significantly between treatment and control groups in the 1st (3.81 times) and 2nd instars (3.82 times). Finally, total lipid content significantly differed between the treatment and control groups of all four instars; treatment groups having 2.04, 1.34, 1.25 and 1.30 times the total lipid content of their respective control groups. [Conclusion] An LC₅₀dose of deltamethrin effects both the growth and nutrient content of all instars of A. solani.

Key words: Acyrthosiphon solani, lethal concentration, deltamethrin, instar sduration, energy substances

高云雷, 韩岚岚, 赵奎军, 张雯林, 肖建飞, 陈娟, 高丽瞳, 邓士群. 致死中浓度溴氰菊酯对茄无网蚜体内能源物质的影响 ^*[J]. 应用昆虫学报, 2020, 57(4): 861-867.

Yun-Lei GAO, Lan-Lan HAN, Kui-Jun ZHAO, Wen-Lin ZHANG, Jian-Fei XIAO, Juan CHEN, Li-Tong GAO, Shi-Qun DENG. Effect of a lethal concentration of deltamethrin on the growth and nutrient content of Acyrthosiphon solani nymphs[J]. Chinese Journal of Applied Entomology, 2020, 57(4): 861-867.

图/表 9

图1 LC50溴氰菊酯对茄无网蚜各虫龄历期的影响柱上标有不同小写字母表示经Student t-检验法检验差异显著（P ? 0.05）。下图同。

Fig. 1 Effect of LC50 deltamethrin on the different instars of Acyrthosiphon solani Histograms with different lowercase letters indicate significant difference at the 0.05 level by Student t- test. The same below.

图2 LC50溴氰菊酯对茄无网蚜蛋白质含量的影响

Fig. 2 Effect of LC50 deltamethrin on the protein content of Acyrthosiphon solani

图3 LC50溴氰菊酯对茄无网蚜蛋白质比重的影响

Fig.3 Effect of LC50 deltamethrin on the protein specific gravity of Acyrthosiphon solani

图4 LC50溴氰菊酯对茄无网蚜可溶性糖含量的影响

Fig. 4 Effect of LC50 deltamethrin on the soluble sugaer content of Acyrthosiphon solani

图5 LC50溴氰菊酯对茄无网蚜可溶性糖比重的影响

Fig. 5 Effect of LC50 deltamethrin on the soluble sugar specific gravity of Acyrthosiphon solani

图6 LC50溴氰菊酯对茄无网蚜糖原含量的影响

Fig. 6 Effect of LC50 deltamethrin on the glycogen content of Acyrthosiphon solani

图7 LC50溴氰菊酯对茄无网蚜糖原比重的影响

Fig. 7 Effect of LC50 deltamethrin on the glycogen specific gravity of Acyrthosiphon solani

图8 LC50溴氰菊酯对茄无网蚜总脂质含量的影响

Fig. 8 Effect of LC50 deltamethrin on the total lipid content of Acyrthosiphon solani

图9 LC50溴氰菊酯对茄无网蚜总脂质比重的影响

Fig. 9 Effect of LC50 deltamethrin on the total lipid specific gravity of Acyrthosiphon solani

参考文献 13

[1]	Birolli WG, Vacondio B, Alvarenga N, Seleghim MHR, Porto ALM , 2018. Enantioselective biodegradation of the pyrethroid (±)-lambda-cyhalothrin by marine-derived fungi. Chemosphere Oxford: S0045653518300626 .
[2]	Cao Y, Liu Y, Yang WJ, Meng YL, Wang LJ, Zeng L, Li C , 2015. The effect of emamectin benzoate exposure on the energy source of Frankliniella occidentalis. Acta Scientiarum Naturalium Universitatis Sunyatseni, 54(6):31-36.
	[ 曹宇, 刘燕, 杨文佳, 孟永禄, 王丽娟, 曾力, 李灿 , 2015. 甲维盐胁迫对西花蓟马能源物质的影响. 中山大学学报(自然科学版), 54(6):31-36.]
[3]	Chen CY, Shi XY, Gao XW , 2016. Mechanism of insect metabolic resistance to pyrethroid insecticides. Chinese Journal of Pesticide Science, 18(5):545-555.
	[ 陈澄宇, 史雪岩, 髙希武 , 2016. 昆虫对拟除虫菊酯类杀虫剂的代谢抗性机制研究进展. 农药学学报, 18(5):545-555.]
[4]	Cui JL, Wang JG, Zhong L, Li WJ , 2018. Effects of mepiquat chloride (DPC) on energy substances in Aphis gossypii. Journal of Environmental Entomology, 40(2):446-451.
	[ 崔家丽, 王俊刚, 钟亮, 李文杰 , 2018. 缩节胺对棉蚜体内能源物质的影响. 环境昆虫学报, 40(2):446-451. ]
[5]	Guo JQ, Zhang MH , 1989. Study on the main mediator of soybean mosaic virus (SMV) and its transmission efficiency. Soybean Science, ( 1):55-63.
	[ 郭井泉, 张明厚 , 1989. 大豆花叶病毒(SMV)主要介体及其传毒效率研究. 大豆科学, ( 1):55-63.]
[6]	Han LL, Wang K, Li DP, Zhang WL, Cheng Y, Zhao KJ , 2016. Effects of the potato-soybean, and maize-soybean, intercropping modes on the population dynamics of main piercing-sucking pests, and other pests, in soybean fields. Chinese Journal of Applied Entomology, 53(4):723-730.
	[ 韩岚岚, 王坤, 李东坡, 张雯林, 程媛, 赵奎军 , 2016. 马铃薯-大豆、玉米-大豆邻作对大豆田主要刺吸式害虫以及其他害虫的种群动态影响. 应用昆虫学报, 53(4):723-730.]
[7]	Lv N , 2015. Studies on the intracellular bacterial symbionts of two color morphs of Acyrthosiphon pisum Harris. Masteral dissertation. Lanzhou: Gansu Agricultrural University.
	[ 吕宁 , 2015. 两种色型豌豆蚜胞内共生菌研究. 硕士学位论文. 兰州: 甘肃农业大学.]
[8]	Tang F, Liang P, Gao XW , 2012. Induction of glutathione S- transferases activity by plant allelochemicals in the cotton bollworn, Helicoverpa armigera. Chinese Journal of Applied Entomology, 49(6):1513-1518.
	[ 汤方, 李丽, 高希武 , 2012. 植物次生物质和杀虫剂对分月扇舟蛾谷胱甘肽S-转移酶的抑制作用. 应用昆虫学报, 49(6):1513-1518.]
[9]	Wang H, Xu ZX, Zhao KJ, Han LL , 2014. Effects of median lethal concentration of imidacloprid on accumulation of energy substances in Aphis glycines(Homoptera: Aphididae). Chinese Journal of Biological Control, 30(5):654-659.
	[ 王红, 徐忠新, 赵奎军, 韩岚岚 , 2014. 致死中浓度吡虫啉对大豆蚜体内能源物质含量的影响. 中国生物防治学报, 30(5):654-659.]
[10]	Wang ZH, Fan JM, Chen JC, Gong YJ, Wei SJ , 2017. Sublethal effects of sulfoxaflor on the growth and reproduction of the green peach aphid Myzus persicae. Scientia Agricultura Sinica, 50(3):496-503. doi: 10.3864/j.issn.0578-1752.2017.03.008 URL
	[ 王泽华, 范佳敏, 陈金翠, 宫亚军, 魏书军 , 2017. 氟啶虫胺腈亚致死浓度对桃蚜生长和繁殖的影响. 中国农业科学, 50(3):496-503.]
[11]	Yin JL, Hu JH, Xu HW, Wu JC, Wang F, Yang GQ , 2008. Comparisons of levels of crude fat, soluble sugars, and free amino acids in offsprings of the immigrant and non-immigrant populations of the brown planthopper, Nilaparvata lugens (Stål)(Homoptera: Delphacidae), under insecticide stress. Acta Entomologica Sinica 51(11):1103-1112.
	[ 印建莉, 胡君欢, 徐海伟, 吴进才, 王飞, 杨国庆 , 2008. 杀虫剂胁迫下褐飞虱迁飞虫和本地虫后代体内粗脂肪、可溶性糖及氨基酸含量的比较. 昆虫学报, 51(11):1103-1112.]
[12]	Zhang J, Qin XW, Yuan FH, Liu J, Huang J, Zhang RJ , 2011. Effects of sub-lethal concentration of nitenpyram on soluble protein, glyceride, free amino acid and soluble sugar of brown planthopper, Nilaparvata lugens (Stål) (Homoptera: Delphacidae). Acta Scientiarum Naturalium Universitatis Sunyatseni 50(6):88-93.
	[ 张洁, 秦小娃, 袁凤辉, 刘杰, 黄婕, 张润杰 , 2011. 亚致死剂量烯啶虫胺对褐飞虱体内脂肪、蛋白质、可溶性糖及游离氨基酸含量的影响. 中山大学学报(自然科学版), 50(6):88-93.]
[13]	Zhang TS, Chang XL, Teng HY, Yuan YD, Wang DS , 2016. Profile of protein and carbohydrate in Spodoptera exigua treated with sublethal concentration of methoxyfenozide. Acta Agriculture Shanghai, 32(6):87-91.
	[ 张天澍, 常晓丽, 滕海媛, 袁永达, 王冬生 , 2016. 亚致死浓度甲氧虫酰肼对甜菜夜蛾蛋白质和糖类含量的影响. 上海农业学报, 32(6):87-91.]