EAG and behavioral responses of Cacia cretifera thibetana to seven walnut tree volatiles

doi:10.7679/j.issn.2095-1353.2020.096

Abstract

Abstract:

[Objectives] To investigate the electroantennogram and olfactory behavioral responses of male and females of the beetle Cacia cretifera thibetana to seven volatile compounds derived from a host plant of this pest, the walnut Juglans sigillata var ‘Santai’. [Methods] Electroantennogram (EAG) and Y-tube olfactometer assays were employed to investigate the responses of C. cretifera thibetana to seven volatile components from J. sigillata var ‘Santai’. [Results] The EAG results show that the tested concentrations of all seven compounds elicited responses from both male and female beetles. The weakest EAG responses were to volatile concentrations of 0.000 4 mol/L and the highest to concentrations of 0.4 mol/L. The strongest EAG responses by males and females, 1.84 and 1.74, respectively, were elicited by nonanal. Y-tube behavioral assays showed that, within a certain range of concentration, six compounds, including alpha-pinene, beta-pinene, 1-caryophyllene, nonaldehyde, eucalyptus oil alcohol and trans-2-hexenal, were attractive to male and female beetles. N-hexyl aldehydes, however, had a repellent effect on both sexes. At a volatile concentration of 2 mol/L, females were most attracted by nonaldehyde whereas males preferred beta-pinene; chemotaxis rates of each sex for each compound were 95% and 87%, respectively. N-hexaldehyde had a repellent effect on both sexes, eliciting chemotaxis rates of 87% and 78%, respectively. [Conclusion] All seven compounds induced an EAG response, and six, including alpha-pinene, bet-pinene, 1-caryophyllene, nonaldehyde, eucalyptus oil alcohol and trans-2-hexenal, were attractive to both sexes. Only one compound, n-hexenal, had a repellent effect. These results provide a foundation for further research on attractants for C. cretifera thibetana.

Key words: Cacia cretifera thibetana, Juglans sigillata var ‘Santai’, volatile compounds, electroantennogram response, Y-type olfactory response

Xiang-Zhong MAO, Bin YANG, Yun-Qiang MA, Ning ZHAO. EAG and behavioral responses of Cacia cretifera thibetana to seven walnut tree volatiles[J]. Chinese Journal of Applied Entomology, 2020, 57(4): 938-945.

Figures/Tables 4

References 33

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化合物名称Compound name	纯度（%）Purity	来源 Source of supply
1-石竹烯 1-caryophyllene	95	Sigma公司
壬醛 Nonanal	≥95	Sigma公司
α-蒎烯 α-pinene	≥98	Sigma公司
β-蒎烯β-pinene	≥97	Sigma公司
桉叶油醇 Cineole	99	上海晶纯试剂有限公司
反式-2-己烯醛 (2E)-hexenal	98	上海晶纯试剂有限公司
正己醛 Hexanal	97	上海晶纯试剂有限公司

化合物名称Compound name	纯度（%）Purity	来源 Source of supply
1-石竹烯 1-caryophyllene	95	Sigma公司
壬醛 Nonanal	≥95	Sigma公司
α-蒎烯 α-pinene	≥98	Sigma公司
β-蒎烯β-pinene	≥97	Sigma公司
桉叶油醇 Cineole	99	上海晶纯试剂有限公司
反式-2-己烯醛 (2E)-hexenal	98	上海晶纯试剂有限公司
正己醛 Hexanal	97	上海晶纯试剂有限公司

化合物Compounds	性别Sex	EAG反应相对值Relative EAG response
化合物Compounds	性别Sex	0.000 4 mol×L^–1	0.004 mol×L^–1	0.04 mol×L^–1	0. 4 mol×L^–1	2 mol×L^–1
α-蒎烯α-pinene	雄性成虫Adult male	0.96±0.01dD	0.98±0.06dD	1.21±0.03cC	1.56±0.04aA	1.32±0.02bB
α-蒎烯α-pinene	雌性成虫Adult female	0.94±0.02 cC	0.94±0.01 cC	0.99±0.02 cC	1.52±0.08 aA	1.31±0.02 bB
β-蒎烯 β-pinene	雄性成虫Adult male	0.95±0.01 cC	1.01±0.04 cC	0.99±0.05 cC	1.44±0.04 aA	1.20±0.07 bB
β-蒎烯 β-pinene	雌性成虫Adult female	0.92±0.01dD	0.97±0.01 cdCD	1.03±0.06cdCD	1.53±0.03aA	1.31±0.04bB
1-石竹烯1-caryophyllene	雄性成虫Adult male	0.89±0.01cC	0.96±0.01cC	1.24±0.07bB	1.72±0.08aA	1.58±0.04aA
1-石竹烯1-caryophyllene	雌性成虫Adult female	0.82±0.01dD	0.94±0.02dD	1.28±0.05cC	1.81±0.07aA	1.49±0.04bB
壬醛Nonanal	雄性成虫Adult male	0.93±0.01dD	0.93±0.01dD	1.22±0.03cC	1.74±0.03aA	1.35±0.03bB
壬醛Nonanal	雌性成虫Adult female	0.94±0.01dD	0.95±0.01dD	1.28±0.01cC	1.84±0.03aA	1.42±0.02bB
桉叶油醇Cineole	雄性成虫Adult male	0.89±0.01deD	0.97±0.01cC	0.95±0.01cdC	1.41±0.03aA	1.20±0.03bB
桉叶油醇Cineole	雌性成虫Adult female	0.88±0.01dD	0.94±0.01cC	0.96±0.01cC	1.43±0.02aA	1.21±0.02bB
反式-2-己烯醛 (2E)-hexenal	雄性成虫Adult male	0.87±0.01eD	0.95±0.01dCD	1.07±0.05cC	1.52±0.02aA	1.39±0.01bB
反式-2-己烯醛 (2E)-hexenal	雌性成虫Adult female	0.89±0.02eC	0.95±0.01dC	1.21±0.02cB	1.40±0.02aA	1.33±0.03aA
正己醛 Hexanal	雄性成虫Adult male	0.87±0.02dC	0.94±0.01cdC	0.97±0.01cC	1.48±0.02aA	1.21±0.05bB
正己醛 Hexanal	雌性成虫Adult female	0.91±0.02dD	0.91±0.01dCD	1.12±0.05cBC	1.54±0.06aA	1.31±0.01bB

化合物Compounds	性别Sex	EAG反应相对值Relative EAG response
化合物Compounds	性别Sex	0.000 4 mol×L^–1	0.004 mol×L^–1	0.04 mol×L^–1	0. 4 mol×L^–1	2 mol×L^–1
α-蒎烯α-pinene	雄性成虫Adult male	0.96±0.01dD	0.98±0.06dD	1.21±0.03cC	1.56±0.04aA	1.32±0.02bB
α-蒎烯α-pinene	雌性成虫Adult female	0.94±0.02 cC	0.94±0.01 cC	0.99±0.02 cC	1.52±0.08 aA	1.31±0.02 bB
β-蒎烯 β-pinene	雄性成虫Adult male	0.95±0.01 cC	1.01±0.04 cC	0.99±0.05 cC	1.44±0.04 aA	1.20±0.07 bB
β-蒎烯 β-pinene	雌性成虫Adult female	0.92±0.01dD	0.97±0.01 cdCD	1.03±0.06cdCD	1.53±0.03aA	1.31±0.04bB
1-石竹烯1-caryophyllene	雄性成虫Adult male	0.89±0.01cC	0.96±0.01cC	1.24±0.07bB	1.72±0.08aA	1.58±0.04aA
1-石竹烯1-caryophyllene	雌性成虫Adult female	0.82±0.01dD	0.94±0.02dD	1.28±0.05cC	1.81±0.07aA	1.49±0.04bB
壬醛Nonanal	雄性成虫Adult male	0.93±0.01dD	0.93±0.01dD	1.22±0.03cC	1.74±0.03aA	1.35±0.03bB
壬醛Nonanal	雌性成虫Adult female	0.94±0.01dD	0.95±0.01dD	1.28±0.01cC	1.84±0.03aA	1.42±0.02bB
桉叶油醇Cineole	雄性成虫Adult male	0.89±0.01deD	0.97±0.01cC	0.95±0.01cdC	1.41±0.03aA	1.20±0.03bB
桉叶油醇Cineole	雌性成虫Adult female	0.88±0.01dD	0.94±0.01cC	0.96±0.01cC	1.43±0.02aA	1.21±0.02bB
反式-2-己烯醛 (2E)-hexenal	雄性成虫Adult male	0.87±0.01eD	0.95±0.01dCD	1.07±0.05cC	1.52±0.02aA	1.39±0.01bB
反式-2-己烯醛 (2E)-hexenal	雌性成虫Adult female	0.89±0.02eC	0.95±0.01dC	1.21±0.02cB	1.40±0.02aA	1.33±0.03aA
正己醛 Hexanal	雄性成虫Adult male	0.87±0.02dC	0.94±0.01cdC	0.97±0.01cC	1.48±0.02aA	1.21±0.05bB
正己醛 Hexanal	雌性成虫Adult female	0.91±0.02dD	0.91±0.01dCD	1.12±0.05cBC	1.54±0.06aA	1.31±0.01bB

化合物Compounds	EAG反应相对值 Relative EAG response
化合物Compounds	雌性成虫 Adult female	雄性成虫 Adult male
α-蒎烯α-pinene	1.56±0.04Ab	1.52±0.08Ab
β-蒎烯β-pinene	1.44±0.04Abc	1.53±0.03Ab
1-石竹烯 1-caryophyllene	1.72±0.08Aa	1.81±0.07Aa
壬醛 Nonanal	1.74±0.03Aa	1.84±0.03Aa
桉叶油醇 Cineole	1.41±0.03Ac	1.43±0.02Abc
反式-2-己烯醛(2E)-hexenal	1.52±0.02A bc	1.40±0.02Ac
正己醛Hexanal	1.48±0.02A bc	1.54±0.06Ab