Chinese Journal of Applied Entomology ›› 2020, Vol. 57 ›› Issue (4): 841-849.doi: 10.7679/j.issn.2095-1353.2020.086
• Special Column • Previous Articles
Dan-Yang GUAN1(), Xiao-Wei JIANG1, Qing-Ya LI1, Xiao LIU1, Yong-Qiang MA2, Qiang CHEN1, Hongmei LI-Byarlay3, Bing-Jun HE1,***()
Received:
2020-01-10
Accepted:
2020-04-09
Online:
2020-07-27
Published:
2020-09-02
Contact:
Bing-Jun HE
E-mail:dy13512892573@163.com;hebj@nankai.edu.cn
Dan-Yang GUAN, Xiao-Wei JIANG, Qing-Ya LI, Xiao LIU, Yong-Qiang MA, Qiang CHEN, Hongmei LI-Byarlay, Bing-Jun HE. Effects of Guadipyr on voltage-gated calcium and potassium channels in central neurons of Helicoverpa armigera[J]. Chinese Journal of Applied Entomology, 2020, 57(4): 841-849.
Fig. 2 TheⅠ-Ⅴcurves and Ipeak of Ca2+ channels before and after the application of Gua A. Gua's effects on the Ⅰ-Ⅴ curve of Ca2+ channels: Standardized current amplitude; B. The Ipeak of Ca2+ channels after Gua. * indicats significant difference compared with the control (P<0.05, One-way analysis of variance). The same as Fig.7.
条件 Condition | 激活 Activation | ||
---|---|---|---|
V0.5a | kb | n | |
对照 Control | –9.83±0.73 | 4.55±0.38 | 10 |
1 μmol?L–1戊吡虫胍 1 μmol?L–1 Gua | –22.85±5.02* | 5.27±0.26 | 5 |
10 μmol?L–1戊吡虫胍 10 μmol?L–1 Gua | –23.45±3.36* | 5.48±0.21 | 8 |
100 μmol?L–1戊吡虫胍 100 μmol?L–1 Gua | –24.21±3.74* | 5.46±0.26 | 5 |
Table 1 Effects of Gua on the steady-state activation of ICa
条件 Condition | 激活 Activation | ||
---|---|---|---|
V0.5a | kb | n | |
对照 Control | –9.83±0.73 | 4.55±0.38 | 10 |
1 μmol?L–1戊吡虫胍 1 μmol?L–1 Gua | –22.85±5.02* | 5.27±0.26 | 5 |
10 μmol?L–1戊吡虫胍 10 μmol?L–1 Gua | –23.45±3.36* | 5.48±0.21 | 8 |
100 μmol?L–1戊吡虫胍 100 μmol?L–1 Gua | –24.21±3.74* | 5.46±0.26 | 5 |
条件 Condition | 失活 Inactivation | ||
---|---|---|---|
V0.5a | kb | n | |
对照 Control | –22.30±2.46 | 18.45±3.60 | 7 |
1 μmol?L–1戊吡虫胍 1 μmol?L–1 Gua | –26.26±2.86 | 13.58±3.36 | 5 |
10 μmol?L–1戊吡虫胍 10 μmol?L–1 Gua | –24.76±2.44 | 12.28±2.95 | 6 |
100 μmol?L–1戊吡虫胍 100 μmol?L–1 Gua | –28.69±3.12 | 13.41±3.13 | 5 |
Table 2 Effects of Gua on the steady-state inactivation of ICa
条件 Condition | 失活 Inactivation | ||
---|---|---|---|
V0.5a | kb | n | |
对照 Control | –22.30±2.46 | 18.45±3.60 | 7 |
1 μmol?L–1戊吡虫胍 1 μmol?L–1 Gua | –26.26±2.86 | 13.58±3.36 | 5 |
10 μmol?L–1戊吡虫胍 10 μmol?L–1 Gua | –24.76±2.44 | 12.28±2.95 | 6 |
100 μmol?L–1戊吡虫胍 100 μmol?L–1 Gua | –28.69±3.12 | 13.41±3.13 | 5 |
Fig. 7 TheⅠ-Ⅴ curves and Ipeak of IK before and after the application of Gua A. Gua’s effects on the I-V curve of K+ channels: Current amplitude is not standardized; B. The Ipeak of K+ channels after Gua.
条件 Condition | 激活 Activation | ||
---|---|---|---|
V0.5a | kb | n | |
对照Control | 22.15±1.72 | 21.10±0.74 | 13 |
10 μmol?L–1戊吡虫胍 10 μmol?L–1 Gua | 31.72±1.07* | 22.31±0.78 | 7 |
100 μmol?L–1戊吡虫胍 100 μmol?L–1 Gua | 29.35±1.55 | 24.16±1.93 | 4 |
Table 3 Effects of Gua on the steady-state activation of IK
条件 Condition | 激活 Activation | ||
---|---|---|---|
V0.5a | kb | n | |
对照Control | 22.15±1.72 | 21.10±0.74 | 13 |
10 μmol?L–1戊吡虫胍 10 μmol?L–1 Gua | 31.72±1.07* | 22.31±0.78 | 7 |
100 μmol?L–1戊吡虫胍 100 μmol?L–1 Gua | 29.35±1.55 | 24.16±1.93 | 4 |
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